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. 2016 Aug 15;16:636. doi: 10.1186/s12885-016-2679-1

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — The detection of 4T1-derived metastatic colonies in the bone marrow is increased in Lgals3−/− mice. Representative immunohistochemical staining of hematoxylin and eosin in the bone marrow of Balb/c a Lgals3+/+ or b Lgals3−/− mice. Representative immunohistochemical staining of CK-19 in the bone marrow of Balb/c c Lgals3+/+ or d Lgals3−/− mice previously inoculated with 105 4T1 mammary carcinoma cells in the fourth mammary fat pad for 28 days. e Number and representative images of clonogenic 4T1 metastatic cells cultured from a total of 105 iliac bone cells 21 and 28 days p.o.i. f CK-19 mRNA levels in iliac bone cells of Balb/c Lgals3+/+ or Lgals3−/− mice 15 days p.o.i. with 4T1 mammary carcinoma cells. Data are the mean ± S.D., n = 3, three animals per group; *p < 0.05, **p < 0.01