Figure 7.

Progressive increases in the levels of specific synaptic proteins in the brain of normal mice between 5 and 15 weeks of age. Quantitative western blot analysis showing significant increases in the level of MeCP2 between 5 and 10 weeks of age, but not between 10 and 15 weeks of age, while the levels of specific synaptic proteins including SYN1, VGLUT1, NMDAR2A, SHANK1 and SHANK2 continue to increase in the brain of 15-week-old wild-type mice when compared with that of 10-week-old wild-type mice. Note that, the levels of other synaptic proteins such as CaMKIIα, CaMKIIβ, GABABR2, GluR2/3, PSD93 and SHANK3 do not change over this period of time. One-way measure ANOVA followed by appropriate post hoc for multiple-comparisons test was used to determine differences. Error bars are mean ± SEM. *P < 0.05 and ^#P < 0.05. n = 3 mice per group.