Figure 4.

Mitochondrial functional parameters in MitoQ- and SS31-treated mutant Htt neurons and in untreated Htt neurons. Mitochondrial function was assessed by measuring: (A) H₂O₂ production, (B) lipid peroxidation, (C) ATP levels, (D) GTPase Drp1 activity and (E) cell viability. Significantly decreased levels were found in the following parameters, in the mutant Htt neurons upon MitoQ and SS31 treatment: H₂O₂ with MitoQ (P = 0.03) and SS31 (P = 0.01); and 4-hydroxy-2-nonenol with MitoQ (P = 0.04) and SS31 (P = 0.01) and GTPase Drp1 activity for MitoQ (P = 0.002) and SS31 (P = 0.005). In contrast, significantly increased levels were found in the following parameters upon MitoQ and SS31 treatment: ATP production with MitoQ (P = 0.04) and SS31 (P = 0.01); and cell viability for MitoQ (P = 0.002) and SS31 (P = 0.005) concentrations.