Table 4.
Summary of antibody dilutions and conditions used in the immunohistochemistry/immunofluorescence analysis of mitochondrial dynamics, synaptic, and DARPP32 proteins in the HDhQ111/Q111 cell lines treated with MitoQ and SS31
| Marker | Primary antibody, species and dilution | Purchased from company, city and state | Secondary antibody, dilution, Alexa fluor dye | Purchased from company, city and state |
|---|---|---|---|---|
| Drp1 | Rabbit Polyclonal 1:300 | Novus Biological, Littleton, CO | Goat anti-rabbit Biotin 1:400, HRP-Streptavidin (1:200), TSA-Alexa488 | KPL, Gaithersburg, MD VECTOR Laboratories Inc., Burlingame, CA Molecular Probe, Grand Island, NY |
| Fis1 | Rabbit Polyclonal 1:300 | Protein Tech Group, Inc., Chicago, IL | Goat anti-rabbit Biotin 1:400, HRP-Streptavidin (1:200), TSA-Alexa488 | KPL, Gaithersburg, MD VECTOR Laboratories Inc., Burlingame, CA Molecular Probe, Grand Island, NY |
| Mfn1 | Rabbit Polyclonal 1:300 | Abcam, Cambridge, MA | Goat anti-rabbit Biotin 1:400, HRP-Streptavidin (1:200), TSA-Alexa594 | KPL, Gaithersburg, MD VECTOR Laboratories Inc., Burlingame, CA Molecular Probe, Grand Island, NY |
| Mfn2 | Rabbit Polyclonal 1:200 | Novus Biological, Littleton, CO | Goat anti-rabbit Biotin 1:400, HRP-Streptavidin (1:200), TSA-Alexa594 | KPL, Gaithersburg, MD VECTOR Laboratories Inc., Burlingame, CA Molecular Probe, Grand Island, NY |
| OPA1 | Mouse Monoclonal 1:500 | BD Biosciences, San Jose, CA | Goat anti-mouse Biotin 1:400, HRP-Streptavidin (1:200), TSA-Alexa488 | KPL, Gaithersburg, MD VECTOR Laboratories Inc., Burlingame, CA Molecular Probe, Grand Island, NY |
| CypD | Mouse Monoclonal 1:500 | EMD, Calobiochem Chemicals Inc., Gibbstown, NJ | Goat anti - mouse Biotin 1:400, HRP-Streptavidin (1:200), TSA-Alexa594 | KPL, Gaithersburg, MD VECTOR Laboratories Inc., Burlingame, CA Molecular Probe, Grand Island, NY |
| SYN | Rabbit Polyclonal 1:400 | Thermo Fisher Scientific Inc., Rockford, IL | Goat anti-rabbit Biotin 1:400, HRP-Streptavidin (1:200), TSA-Alexa488 | KPL, Gaithersburg, MD VECTOR Laboratories Inc., Burlingame, CA Molecular Probe, Grand Island, NY |
| PSD95 | Rabbit Monoclonal 1:300 | Abcam, Cambridge, MA | Goat anti-rabbit Biotin 1:400, HRP-Streptavidin (1:200), TSA-Alexa488 | KPL, Gaithersburg, MD VECTOR Laboratories Inc., Burlingame, CA Molecular Probe, Grand Island, NY |
| DARPP32 | Rabbit Polyclonal 1:200 | Santa Cruz Biotechnology, Inc., Dallas, TX | Goat anti-rabbit Biotin 1:400, HRP-Streptavidin (1:200), TSA-Alexa488 | KPL, Gaithersburg, MD VECTOR Laboratories Inc., Burlingame, CA Molecular Probe, Grand Island, NY |