Figure 5.

Motor neuron differentiation of Slc52a3 KO ES cells. (A–C) qPCR assay of Slc52a3, Oct-3/4 and Isl1 were performed through the differentiation into motor neurons at Day 0, 7 and 14. *P < 0.05; **P < 0.01, N.S.: no significance, ND: not detected. All expression levels were normalized to GAPDH. (D) Motor neurons derived from ES cells from each genotype were stained with anti-Isl1/2 and Tuj1. (E) Percentage of motor neurons from each culture was determined by quantification of Isl1/2 / Tuj1 positive cells. Bars: 200 μm.