Slc52a3 expression in adult tissues. (A) qPCR assay of Slc52a3 expression in adult tissues. Expression levels were normalized by GAPDH. (B) Western blotting of adult tissues. (C–E) Immunofluorescent staining and X-gal staining of anti-Slc52a3 in intestine (C), testis (D) and seminal vesicle (E). V, intestinal villus; IC: intestinal crypt; S, seminiferous tubule; L, Leydig cells; CT, connective tissue; MM, mucous membrane. Bars: 200 μm.