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. 2016 Aug 16;9:63. doi: 10.1186/s13039-016-0263-7

Table 1.

Summary of Target FISH results on 40 patients

No. Sex/Age PC%a BioViewb Del(17p)/TP53 t(4;14) t(14;16) +1q21 Others
1 M/52 26 % 14–16 % (10323–15875) Neg (230)c 38 % + (74) Neg (201) 30 % + (180)
2 F/80 15 % 3–4 % (9605–13508) Neg (89) Neg (61) Neg (71) Neg (71) Normal cytogenetics
3 M/69 12 % 3–4 % (8880–11094) Neg (84) Neg (46)g Neg (40)g Neg (62)
4 M/81 43 % No record Neg (253) Neg (188) Neg (175) Neg (200) 5/9/15 60 % + (235)
5 M/64 15 % 58–64 % (11912–17007) Neg (159) Neg (153) Neg (156)g Neg (155) 5/9/15 Neg (84)
6 M/70 17 % 4–7 % (7132–7616) Neg (63) Neg (53)g Neg (51)g 80 % + (53) 5/9/15 Neg (61)
7 F/52 35 % 8–12 % (7750–8981) Neg (159) 90 % + (112)g Neg (102)g 65 % + (167) Cytogeneticsd: hypodiploid 78.5 %, hyperdiploid 7 %, normal 14.5 %
8 F/72 13 % 42–55 % (11829–14627) Neg (32) Neg (35) Neg (71) Neg (54)
9 F/71 17 % 7–9 % (8667–11174) Neg (82) 70 % + (54) Neg (37) 50 % + (65) Normal cytogenetics
10 M/61 65 % 17–24 % (10289–13997) Neg (135) Neg (94)g Neg (98)g 90 % + (145)
11 M/54 15 % 15–22 % (12898–19268) Neg (141) 80 % + (87) Neg (84) Neg (113) 5/9/15 Neg (171)
12 M/47 100 % 50–59 % (10424–12419) 50 % + (132) Neg (144)g Neg (107)g Neg (156)
13 M/59 60 % 10–12 % (9514–13412) Neg (154) Neg (133)g Neg (128)g Neg (140) t(11;14) 90 %+; normal cytogenetics
14 M/81 22 % 50–52 % (10110–15964) Neg (140) 60 % + (78)g Neg (111)g 85 % + (172) 5/9/15 Neg (101)
15 F/67 45 % 21–27 % (6066–7366) Neg (163) 90 % + (145) Neg (136) 85 % + (183) 5/9/15 Neg (157)
16 M/58 No record 8–9 % (6666–8923) Neg (250) Neg (192)g Neg (188)g Neg (224) t(11;14) 25 % + (228)
17 F/70 51 % 13–17 % (7469–8551) Neg (183) Neg (137)g Neg (118)g 85 % + (162) Normal cytogenetics
18 F/53 23 % 10 % (2719–4195) Neg (198) Neg (146) Neg (80) Neg (106) 5/9/15 Neg (87)
19 M/76 70 % 6–7 % (4145–5686) Neg (108) Neg (89) Neg (102) Neg (101) IgH Neg (71)
20 M/61 49 % 17–19 % (4210–5352) Neg (170) Neg (126) Neg (119) 85 % + (172)
21 M/61 20 % 24–29 % (9895–11736) Neg (145) Neg (122) Neg (87) Neg (142) Normal cytogenetics
22 F/48 59 % 6–9 % (6942–7583) 40 % + (172) 65 % + (110) Neg (111) 55 % + (154) Normal cytogenetics
23 F/56 100 % 3–8 % (4400–6203) Neg (123) Neg (149)g Neg (118) 65 % + (158)
24 F/55 84 % 3–6 % (9304–10362) Neg (141) Neg (148) Neg (107) Neg (82) t(11;14) 85 % + (121)
25 M/52 74 % 11–23 % (10024–12394) Neg (158) 85 % + (100) Neg (135)g 90 % + (162) Cytogeneticse: hypodiploid clone, der(4)t(1;4)(q21;p16) and add(7p)
26 F/56 35 % 11–17 % (10247–12375) Neg (100) Neg (166) Neg (140)g Neg (117)
27 F/55 73 % 10–17 % (3419–5581) Neg (186) Neg (167) Neg (142) Neg (171)
28 F/57 15 % 6–8 % (5191–6330) Neg (102) Neg (122) Neg (102) Neg (100)
29 F/69 16 % 25–32 % (7923–12371) Neg (172) Neg (143)g Neg (104)g Neg (173)
30 M/58 19 % 3–5 % (6385–8924) Neg (213) Neg (169) Neg (154) Neg (208)
31 F/66 29 % 7–11 % (7383–9153) Neg (149) 70 % + (73) Neg (108)g 80 % + (127)
32 F/56 15 % 7–12 % (11256–13079) Neg (146) Neg (117)g Neg (93) Neg (150)
33 M/64 13 % 4–6 % (11082–12940) Neg (118) Neg (100) Neg (98) Neg (101) t(11;14) 40 % + (149); cytogeneticsf: loss of Y chromosome
34 F/67 21 % 6–13 % g11872–14598) Neg (159) 70 % + (104) Neg (118)g 95 % + (136)
35 F/89 12 % 3–5 % (11793–14289) Neg (121) Neg (134) Neg (102) Neg (102) Normal cytogenetics
36 F/50 56 % 14–17 % (10614–13489) Neg (140) Neg (143) Neg (111) 60 % + (137)
37 M/60 50 % 24–27 % (10633–13622) Neg (165) Neg (121) Neg (117) 83 % + (143)
38 M/79 20 % 6–10 % (13896–15969) Neg (125) Neg (96) Neg (121)g 80 % + (144) 80 % trisomy TP53
39 F/67 53 % 32–38 % (13807–17047) Neg (162) 63 % + (123) Neg (103) 63 % + (104)
40 M/69 57 % 6–13 % (12778–15272) Neg (158) Neg (127) Neg (148) Neg (158)

Key:

aThe PC% refers to the plasma cell percentage in the bone marrow aspirate as enumerated on microscopic examination

bThe BioView data refers to the percentage of cells in the plasma cell category as recognized by the automated image capture and analysis system before manual re-classification. The number in the parentheses refers to the total number of cells analyzed by the automated image capture and analysis system on the slide. Both are reported as a range of figures obtained from the 4 slides

cThe number in parenthesis is the actual number of re-classified plasma cells with optimal FISH signals on which the positive or negative result is based. This figure is also indicated on the pathology report for each FISH probe

d26 ~ 45,XX,t(4;14)(p16;q32)[33]/48 ~ 159,XX[3]/46,XX[6]

e43 ~ 44,XY,+3[2],der(4)t(1;4)(q21;p16)[2],add(7)(p13)[2],+7 ~ 8mar[2][cp2]/46,XY[4]

f45,X,-Y[3]/46,XY[15]

gDenotes the incidental finding of secondary abnormalities i.e. monosomy of deletion of chromosomes 4, 14 or 16; trisomy or amplification of chromosomes 4, 14 or 16