Table V.
The When. Replies to questions for embryologists.
| Answer | A.D.V. | G.K. | J.H. | M.Me. | M.Mo. | L.R. | K.Sc. | J.S. |
|---|---|---|---|---|---|---|---|---|
| Q1: Which type of DNA source material do you use for PGS? | ||||||||
| Polar body | X | X | X | |||||
| Blastomere | X | |||||||
| Trophectoderm | X | X | X | X | X | X | X | |
| Blastocoelic fluid | – | – | – | – | – | – | – | – |
| Q2: If applicable, which method of biopsying both polar bodies, do you prefer? | ||||||||
| Simultaneous | X | X | X | |||||
| Sequential | X | X | ||||||
| Q3: At what time after ICSI do you perform the biopsy(ies)? | ||||||||
| Polar body | 9 h | 9–10 h | 12–16 h | 7–9 h | ||||
| Blastomere | 70 h | 64–72 h | 64–72 h | 68–70 h | ||||
| Trophectoderm | D5, D6 | 106–110 h | 115 h | 116–120 h | D5–7 | 112–150 h | 118–142 h | |
| Blastocoelic fluid | – | – | – | – | – | – | – | – |
| Q4: Which method do you use for zona breaching? | ||||||||
| Laser | X | X | X | X | X | X | X | X |
| Mechanical | – | – | – | – | – | – | – | – |
| Acid Tyrode's | – | – | – | – | – | – | – | – |
| Q5: In which culture medium do you biopsy? * With HEPES added; ° Only for blastomere biopsy | ||||||||
| Standard culture medium | X | X | X* | X | X* | X | X | X |
| Ca2+-Mg2−-free° | X | X | X | X | ||||
| Q7: What do you do with undiagnosed embryos? See text for more details | ||||||||
| Discard | – | – | – | – | – | – | – | – |
| Transfer | X | X | ||||||
| Rebiopsy | X | X | X | X | X | X | ||
| Q8: Are you involved in one or more PGS trial? Type of trials are detailed in Table III. | ||||||||
| Yes | X | X | X | X | ||||