Table III.

Quantitative RT–PCR validation of selected genes.

Gene name	Model	Quantitative RT–PCR fold change	Quantitative RT–PCR direction of change	Microarray fold change	Microarray direction of change
PTGS2	Explants	7.02 ± 3.34	↑	24.55 ± 0.45	↑
	Myometrial cells	2.43 ± 0.65	↑	2.21 ± 0.41	↑
	hTERT	1.73 ± 0.29	↑	2.47 ± 0.02	↑
IL8	Explants	200.53 ± 43.89	↑	28.53 ± 0.45	↑
	Myometrial cells	−4.51 ± 0.07	↓	−1.22 ± 0.14	↓
	hTERT	14.26 ± 0.05	↑	9.92 ± 0.46	↑
OXTR	Explants	−11.93 ± 0.05	↓	−1.23 ± 0.21	↓
	Myometrial cells	−34.74 ± 0.01	↓	−7.74 ± 0.42	↓
	hTERT	−9.09 ± 0.02	↓	−3.31 ± 0.20	↓
PGR	Explants	−2.89 ± 0.04	↓	−1.76 ± 0.08	↓
	Myometrial cells	−4.43 ± 0.04	↓	−3.35 ± 0.45	↓
	hTERT	−18.47 ± 0.00	↓	−8.21 ± 0.08	↓
IL1B	Explants	2.53 ± 0.09	↑	2.64 ± 0.40	↑
	Myometrial cells	−35.06 ± 0.01	↓	−1.31 ± 0.11	↓
	hTERT	18.90 ± 0.80	↑	18.44 ± 0.36	↑
NR3C1	Explants	−2.19 ± 0.04	↓	1.39 ± 0.03	↑
	Myometrial cells	−1.64 ± 0.08	↓	1.28 ± 0.14	↑
	hTERT	−1.23 ± 0.04	↓	1.81 ± 0.04	↑
TPM1	Explants	−1.37 ± 0.10	↓	−4.40 ± 0.05	↓
	Myometrial cells	−3.05 ± 0.04	↓	−2.77 ± 0.16	↓
	hTERT	1.33 ± 0.06	↑	−1.48 ± 0.08	↓
TNFAIP3	Explants	4.09 ± 1.30	↑	6.44 ± 0.19	↑
	Myometrial cells	−13.44 ± 0.01	↓	−5.92 ± 0.12	↓
	hTERT	−2.99 ± 0.04	↓	−1.08 ± 0.12	↓
IL6	Explants	20.12 ± 4.27	↑	15.21 ± 0.21	↑
	Myometrial cells	1.66 ± 0.46	↑	2.03 ± 0.31	↑
	hTERT	8.32 ± 1.59	↑	9.47 ± 0.11	↑
ACTA2	Explants	−10.48 ± 0.03	↓	−1.79 ± 0.14	↓
	Myometrial cells	−15.08 ± 0.02	↓	−1.49 ± 0.24	↓
	hTERT	−44.47 ± 0.00	↓	−2.83 ± 0.07	↓
MYLK	Explants	−8.98 ± 0.02	↓	−1.40 ± 0.03	↓
	Myometrial cells	−13.14 ± 0.01	↓	−1.14 ± 0.19	↓
	hTERT	−124.56 ± 0.00	↓	−3.58 ± 0.06	↓
PTGER3	Explants	−3.59 ± 0.09	↓	−1.10 ± 0.08	↓
	Myometrial cells	−10.37 ± 0.03	↓	−5.57 ± 0.21	↓
	hTERT	−653.11 ± 0.00	↓	−24.47 ± 0.04	↓
GJA1	Explants	1.06 ± 0.18	↑	1.58 ± 0.10	↑
	Myometrial cells	2.10 ± 0.19	↑	2.56 ± 0.11	↑
	hTERT	1.49 ± 0.11	↑	1.39 ± 0.03	↑
ESR1	Explants	−1.58 ± 0.10	↓	1.30 ± 0.17	↑
	Myometrial cells	1.41 ± 0.23	↑	−1.01 ± 0.13	↓
	hTERT	−3.56 ± 0.02	↓	−1.32 ± 0.08	↓
IL1A	Explants	3.36 ± 0.83	↑	2.71 ± 0.35	↑
	Myometrial cells	−1.55 ± 0.04	↓	−1.26 ± 0.10	↓
	hTERT	18.24 ± 3.60	↑	17.09 ± 0.19	↑

Myometrial tissue obtained from non-labouring women at term was snap frozen at the time Caesarean section (t = 0), finely dissected into 3 × 3 × 3 mm³ explants or digested with a collagenase mixture to isolate the myometrial cells. Explants were left in culture without treatment for 30 h. After overnight incubation with 1% charcoal and dextran-stripped fetal calf serum, the media of myometrial cells at passage 4 and hTERT myometrial cells was refreshed and cells were left in culture for 30 h without treatment. RNA was extracted, cDNA was synthesized and quantitative RT–PCR was performed as described in Methods. Data presented as mean fold change ± SEM; n = 6.