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. 2016 Jun 30;60(1):9–18. doi: 10.1042/EBC20150002

Figure 2. Recombinant antibody formats.

Figure 2.

A scFv consists of the variable heavy (VH) and variable light (VL) regions of an Ab joined via a peptide linker. The Fab fragment is composed of VH and VL domains within both constant heavy and light chains. F(ab’)2 involves linking two Fab Abs by disulphide bonds. A Diabody is generated when the variable domains from two scFv Abs are expressed as two polypeptide chains with the domains connected by a short polypeptide linker. A dimeric scFv is generated by the fusion of two scFv Ab via a naturally occurring dimeric protein. A nanobody is an Ab fragment consisting of a single monomeric variable Ab domain. (Adapted from [1].)