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. 2016 Jul 25;213(8):1537–1553. doi: 10.1084/jem.20150577

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© 2016 Parsa et al.

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Figure 4. — Enhanced neutrophil recruitment to the draining iLN of LysM-DTA mice after CFA immunization. (A and B) Flow cytometry analysis of percentages and total numbers of neutrophils (CD11b⁺Ly6G⁺) in the blood or BM (A) and in the iLN (B) after s.c. immunization with CFA. (C) Quantitative RT-PCR analysis of CXCL1, CXCL2, COX1, and COX2 expression (expr.) in lymphocytes at day 7 p.i. Expression is normalized to HPRT. (D) Immunohistochemistry visualizing neutrophils (Ly6G⁺, red) in the cortex (C) or paracortex (PC) of iLNs at 2 h or 14 d p.i. B cells: B220, green; T cells: CD3, blue. (E) Analysis of receptor expression on neutrophils in iLNs at day 7 after CFA. Cells were gated on CD11b⁺Ly-6G⁺ cells. MFI, mean fluorescence intensity. Data in A, B, C, and E are representative of three independent experiments. n = 4–5/group. Results are mean ± SEM. The images in D are representative of four separate mice. Bar, 100 µm. *, P < 0.05; **, P < 0.01 (unpaired Student’s t test).