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. 2016 Jul 25;213(8):1537–1553. doi: 10.1084/jem.20150577

Figure 5.

Figure 5.

Profound number of plasma cells and increased IL-17–producing T cells in LysM-DTA mice. (A) The frequency of proliferating (prolif.) T cells (CD3+Ki67+), regulatory T cells (CD3+CD4+FoxP3+; Tregs), and activated CD4 T cells (CD62L−CD44hi) in the iLN from LysM-DTA and WT mice. (B) IL-17– and IFN-γ–producing CD4+ T cells in WT and LysM-DTA iLNs at 7 and 14 d after CFA immunization. (C) Analysis of B cells (B220+CD138−) and plasma cells (B220−CD138+) in the iLN at 7 and 14 d. (D) Analysis of B cells (B220+), plasma cells (B220−CD138+), neutrophils (SSC+CD11b+Ly6Cint), and IFN-γ/IL-17–producing T helper cells (CD3+CD4+) in the iLN in WT mice treated with neutrophil-depleting anti-Ly6G (1A8) or isotype control (2A3) antibodies (Ab). Mice were injected i.p. with 500 µg of antibody on days −1, 1, 2, 5, 8, 11, and 13 and sacrificed for analysis on day 14 p.i. Data in A–C are representative data from three independent experiments. n = 4–5/group. Data in D are representative of two independent experiments. n = 5/group. Results are mean ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (unpaired Student’s t test).