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. 2016 Jul 25;213(8):1589–1608. doi: 10.1084/jem.20151467

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© 2016 Ma et al.

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Figure 3. — Differential induction of STAT activation by in vitro–polarizing conditions. Naive CD4⁺ T cells were cultured with TAE beads for 4 d. After this time, the cells were harvested, and then cultured in media alone (blue histogram) or under Th1 (+IL-12), Th2 (+IL-4), or Th17 (+TGF-β, IL-1β, IL-6, IL-21, IL-23, and PGE2) conditions, or with IL-10, IL-21, or IFN-γ (red histograms) for 30 min. Phosphorylation of STAT1, STAT3, STAT4, and STAT6 was determined by intracellular staining and flow cytometry. The data are representative of experiments performed on cells from two to three different donors.