Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jul 25;213(8):1589–1608. doi: 10.1084/jem.20151467

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Ma et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 4. — Impact of disease-causing mutations on differentiation of naive CD4⁺ T cells to Th1, Th2, Th17, and Tfh fates in vitro. Naive CD4⁺ T cells were isolated from peripheral blood of healthy donors or patients with mutations in STAT3, STAT1, IL21/R, IL10R, CD40LG, NEMO, BTK, ICOS, IL12RB1, IFNGR1, or TYK2, and then cultured with TAE beads alone (Th0) or under Th1/Tfh (+IL-12), Th2 (+IL-4), or Th17 (+TGF-β, IL-1β, IL-6, IL-21, and IL-23) conditions for 5 d. Cells were then harvested and analyzed for expression of cytokines and transcription factors. (A) Percentage of cells expressing IL-21 in response to Tfh/IL-12 stimulation. (B) Percentage of cells expressing IL-2 in Th0 culture. (C and D) Induction of TBX21 (C) and IFN-γ (D) expression after Th1/IL-12–polarizing culture. (E) Induction of GATA3 in response to Th2/IL-4 stimulation. (F) Induction of IL-17A and IL-17F secretion after Th17-polarizing culture. Controls, n = 17–48; STAT3_DN, n = 2–10; STAT1_GOF, n = 5–14; STAT1_LOF, n = 3–8; IL21R, n = 3–5 experiments; IL21 n = 1; IL10R, n = 1–2; CD40LG, n = 3–9 (CD40LG* _(MILD), n = 3); NEMO, n = 2–7; BTK, n = 5–8; ICOS, n = 2–7; IL12RB1, n = 3–8; IFNGR1, n = 2–6; TYK2, n = 2–3. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001, compared with Th0 (ANOVA; Student’s t test). The dashed lines indicate the mean percentage of IL-21⁺ (A) or IFN-γ⁺ (D) cells, or secretion of IL-17A/F (F), detected under Th0 conditions. Note that data for IL-17A/F production by TYK2-deficient naive CD4⁺ T cells was presented in Kreins et al. (2015), but is shown here for comparison.