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. 2014 Oct 8;24(4):1061–1076. doi: 10.1093/hmg/ddu520

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© The Author 2014. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Immunoblots in protein extracts from brain hemisphere at the age of 1 year. Quantitative comparison of proteins with soluble (RIPA fraction) or more insoluble (SDS fraction) distribution, in brain hemispheres from WT (F1-hybrids between FVB/N and 129/SvEv), both SM lines and DM mice. Altered abundance of p62 could not be detected in any condition; beta-actin was used as a loading control. (A) RIPA fraction shows the increased dosage of alpha-synuclein owing to the overexpression of human A53T-SNCA in the DM and the PrPmtA mice, with the appearance of phospho-Serine129-alpha-synuclein immunoreactivity (pSer129) in these two lines. (B) SDS fraction confirms the increased abundance of alpha-synuclein owing to the presence of human A53T-SNCA in these two lines and documents the appearance of additional pSer129 immunoreactive bands of higher molecular for both lines. The antibody against the alpha-synuclein C-term epitope detected a putative light increase of immunoreactivity in DM tissue.