Table 1.
Summary of variables and virus detection with RT‐qPCR (expressed as log10 EID50 equivalents/m2 of wall or m3 of air) in the different dust samples p.i of the four replicate experiments. Samples not available are ‘na’
| Trial | Dust sampling | Virus titre (log10 EID50/m2 or/m3) | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Room | Apparatus | Sampling time (minutes) | Area (m2) | Number of infected birds | Number of non‐infected birds | Days post‐inoculation | ||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 13 | 14 | |||||||
| 1 | Inoculated | 48 | 0 | |||||||||||||||
| Recipient | MD8 | 90 | 0 | 14 | 0 | 2·9 | 2·1 | 3·1 | 2·6 | 2·8 | 2·9 | 2·7 | 0 | 0 | na | na | ||
| 2 | Inoculated | 35 | 1 | |||||||||||||||
| Recipient | Andersen – non‐respirable | 90 | 14 | 0 | 0 | 0 | 0 | 2·3 | 0 | 1·5 | 0 | 0 | 0 | 0 | na | na | ||
| Recipient | Andersen – respirable | 90 | 0 | 0 | 0 | 0 | 2·0 | 0 | 0 | 0 | 2·2 | 2·8 | na | na | ||||
| 3 | Inoculated | Dust cloths | 0·1 | 30 | 7 | 0 | 3·9 | 5·0 | 3·7 | 4·9 | 5·1 | 4·8 | 4·9 | 4·8 | 4·4 | 4·9 | 3·5 | |
| Recipient | Dust cloths | 0·1 | 0 | 14 | 0 | 2·9 | 4·0 | 3·9 | 3·9 | 4·5 | 4·0 | 4·4 | 4·5 | 3·9 | 3·8 | 2·7 | ||
| Recipient | Andersen – non‐respirable | 270 | 0 | 0 | 0 | 2·5 | 0 | 3·1 | 0 | 3·1 | 2·4 | 2·5 | 2·7 | 3·4 | ||||
| Recipient | Andersen – respirable | 270 | 0 | 2·4 | 0 | 2·9 | 2·7 | 0 | 0 | 3·3 | 2·5 | 3·2 | 2·5 | 2·8 | ||||
| 4 | Inoculated | Dust cloths | 0·1 | 47 | 0 | 0 | 3·3 | 4·5 | 4·6 | 4·1 | 4·6 | 4·9 | 4·8 | 5·0 | 4·9 | 5·4 | 3·9 | |
| Recipient | Dust cloths | 0·1 | 14 | 0 | 0 | 0 | 3·5 | 3·2 | 3·9 | 4·1 | 4·7 | 4·8 | 3·9 | 4·2 | 4·7 | 3·5 | ||
| Recipient | MD8 | 270 | 0 | 2·5 | 2·9 | 3·5 | 3·2 | 4·1 | 3·9 | 3·5 | 4·0 | 3·7 | 3·5 | 3·7 | ||||