Figure 5.

c-Jun regulates hepatic Opn expression. (a and b) Hepatic Opn expression was determined at the indicated time points by qPCR (a) and ELISA (b), respectively. Opn RNA expression was normalized to expression in HBV^- controls. (n≥4 per genotype and time point, significance was tested by Mann–Whitney test (MWT)). (c) Opn expression in primary hepatocytes was determined by qPCR and ELISA from the supernatant, respectively. (n≥4 per genotype, significance was tested by MWT). (d) Expression analysis of Opn, Sox9 and CK19 by immunohistochemistry. Hepatocytes, bile duct epithelia and proliferative ductular cells are indicated by black, blue and red arrows, respectively