Figure 5.

Bcl-2 and TRAIL determine FOXO3/EtOH-induced apoptosis. (a) Western blots in Huh7.5 cell lysates demonstrating siRNA-induced TRAIL knockdown efficiency and ability to restore EtOH-induced loss of Bcl-2 protein through Bcl-2 overexpression. (b and c) LDH release and caspase 3/7 activity induced by FOXO3 expression and EtOH treatment in cells that are deficient in TRAIL (black bars) or in cells in which Bcl-2 levels were maintained through Bcl-2 overexpression (gray bars). (d) Western blot to assess siRNA knockdown efficiency of Bcl-2. (e and f) LDH release and caspase 3/7 activity induced by exogenous TRAIL (100 ng/ml, black bars) and EtOH (gray bars) in cells that are deficient in FOXO3 or Bcl-2. Values are mean±S.D. of three independent experiments. **P<0.01, ***P<0.001, Student's t-test