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. 2015 Oct 9;23(4):628–639. doi: 10.1038/cdd.2015.130

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mi-shRNA transgene expression is fully reversible in DTrA1 mice. Evaluation of transgene induction, as measured by appearance of GFP⁺⁺ cells by flow cytometry in double-transgenic DTrA1 mice fed with doxycycline-containing food for 17 days. Peripheral blood was analyzed at the indicated time-points and cells were co-stained for markers specific for (a) myeloid cells (Gr-1⁺Mac-1⁺), (b) T (pooled CD4⁺ and CD8⁺) or (c) B cells (CD19⁺). Cells were gated on forward/side scatter, lack of Annexin V binding, lineage markers and GFP. Cells were categorized in three groups: GFP⁺⁺, GFP⁺ and GFP^-. Symbols represent means±S.E.M. (ON DOX: n=8, OFF DOX: n=5)