Figure 4.

Increased cardiomyocyte apoptosis in Gab1-cKO mice in response to pressure overload. (a and b) Increased apoptosis of cardiomyocytes. TUNEL-positive cardiomyocytes (WGA staining for cardiomyocyte membrane) from the heart histological sections of WT and Gab1-cKO mice after 2 weeks of TAC or sham operation. (c and d) Decreased ratio of Bcl-2/Bax mRNA. The levels of anti-apoptosis gene Bcl-2 and pro-apoptosis gene Bax expression after 2 weeks of TAC or sham operation were analyzed by semiquantitative RT-PCR. Two individual heart samples from each group were presented in each gel; n=4 hearts for each group. *P<0.05; **P<0.01