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. Author manuscript; available in PMC: 2016 Aug 16.
Published in final edited form as: J Mol Med (Berl). 2012 Jan 7;90(6):707–718. doi: 10.1007/s00109-011-0851-2

Figure 1. Overexpression of AE2 was correlated with cell proliferation in colon cancer.

Figure 1

(A) AE2 mRNA was analyzed by RT-PCR (a, upper right corner: 1, adjacent normal tissue; 2, colon cancer tissue; upper bands, AE2, lower bands, GAPDH). AE2 protein was analyzed by immunohistochemistry. The arrowed profiles in (a) were enlarged in (b, black arrow) and (c, red arrow). Original magnifications ×40 in a, x400 in b and c. (B) AE2 expression in para-cancer and colon cancer tissues was statistically analyzed. (C) Co-localization of AE2 and Ki67 in colon cancer tissues detected by immunohistochemistry. The arrowed profiles in (a) were enlarged in (c, black arrow) and (e, red arrow). The arrowed profiles in (b) were enlarged in (d, black arrow) and (f, red arrow). Original magnifications x200 in a and b, x1000 in c, d, e and f. (D) Association of histochemically detected expression of AE2 and Ki67 in colon cancer sections. (E) Growth curves of SW1116 cells transfected with siRNA-AE2 or siRNA-NC (NSC). Inset, immunoblot analysis of AE2 and cyclin D1. * P<0.05 (n=3). (F) Growth curves of SW1116 cells transfected with pEGFP-AE2 or pEGFP-C1 empty vector (control). Inset, immunoblot analysis of AE2 and cyclin D1. * P<0.05 (n=3).