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. 2015 Sep 3;5:12820. doi: 10.1038/srep12820

Figure 1. HTPCs secrete PEDF in vivo and in vitro and PEDF is regulated by DHT.

Figure 1

(A) Immunohistochemistry using a human testicular biopsy reveals PEDF in the peritubular compartment, in cells and extracellular matrix (arrows point at peritubular cells). Hematoxylin counterstain of cell nuclei. (B) Preadsorption control and (C) negative control (goat normal serum instead of first antibody); each bar represents 10 μm. Hematoxylin counterstain of cell nuclei. (D) RT-PCR and Western Blot experiments confirmed PEDF expression in HTPCs. (E) Summary of qPCR studies showing significantly increased mRNA levels of PEDF when HTPCs were treated with 10 μM DHT for 3d (n = 3). Dot plots depict the individual levels, which are normalized to the solvent ethanol, based on calculated Ct values of treatment and the housekeeping gene L19. p < 0.05 (*).