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. 2015 Sep 3;5:12820. doi: 10.1038/srep12820

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Copyright © 2015, Macmillan Publishers Limited

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(A) RT-PCR analysis revealed the presence of a PEDF receptor (PNPLA2) mRNA and a specific PNPLA2 antibody detected PNPLA2 protein expression in HUVECs. (B) The results of a viability assay based on the levels of ATP indicate that PEDF (10 nM and 100 nM) does not influence the ATP concentration within 24 h, whereas the positive control, 1 nM Staurosporin (SP) significantly reduced viability. Number of replicates is n = 6. One-way ANOVA, p < 0.05 (a versus b). Bars are means +/− SEM.