Figure 2.

HNN cells show a decreased migratory response to an electric field. (A-D) The paths of migration of WT and HNN cells in the presence and absence of an electric field. The x-axis shows the movement along the electric field axis, with the negative values showing movement toward the cathode and the positive values toward the anode. The y-axis shows displacement in the plane perpendicular to the electric field. The intersection of the 2 axes denotes the starting position of each cell, the lines show the path taken by it and the dot indicates the cells position at the end of the experiment. Black denotes cells showing net migration to the cathode, while red those to the anode. (E-F) Show graphs of the various parameters of cell migration. Panel A shows the x-Forward Direction Index, which measures the direction of cell migration with respect to the DCEF. There is a general effect of the electric field on both cell types (p < 0.01), as well as an effect for each of the specific lines (WT, p < 0.05; HNN, p < 0.05). Panel (F) shows the Velocity for each cell line, defined as displacement from the point of origin in time. The DCEF had a significant effect on the WT cells (p < 0.05); there was also a significant difference between WT and HNN cells at 400 mV/mm (p < 0.01) and a general difference between both cell lines (p < 0.05).