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. 2016 Aug 5;57(10):4035–4044. doi: 10.1167/iovs.16-19073

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Retinal histopathology and mtDNA damage in ZDF rats: (a) trypsin-digested retinal microvessels from 12- and 40-week-old ZDF rats were stained with periodic acid Schiff-hematoxylin for acellular capillaries and the arrow indicates an acellular capillary; the picture shows microvasculature from 12- and 40-week-old ZDF rats and 40-week-old lean rats; (b) mtDNA damage in the retina was determined by extended-length PCR. Values are represented as mean ± SD from five to seven rats per group; *P < 0.05 compared with age-matched lean rats. Measurements were performed on five to six rats in each group.