Fig. 4.

Large FOV imaging of the EmSight. The sample is mouse ventral midbrain cultures. (A) Full FOV FPM reconstructed phase image. The size of FOV is 5.7 mm × 4.3 mm. (B) Images of a dopaminergic neuron (DA neuron) from conventional microscope (20X/NA0.4). (B1) Fluorescence image, and (B2) phase contrast image. (C-E) Enlarged images at a distance of 1.8 mm, 2.2 mm, and 3.4 mm away from the center of the FOV, respectively. (C1-E1) Raw images overlaid with fluorescence images (Green color). In this GENSAT strain, dopaminergic neurons produce the eGFP signal. (C2-E2) The reconstructed phase images. Neurites of the neuron which are not distinguished in the raw images are clearly visible in the reconstructed phase images (red arrows).