Fig. 3.
Anthocyanin accumulation in leaves of phosphorus-limited seedlings. Five-day-old seedlings were grown on low-phosphate (Pi) medium for 4 d before being transferred to plates with minimal Pi- (5 μM, white bars), high Pi- (250 μM, black bars), or phosphite- (250 μM, grey bars) containing media. Leaf anthocyanin concentrations were determined at day 1, 2, 3, and 7 after transfer (mean ±SE, n=3 replicates with 12 seedlings each). Statistically significant differences between time points and treatments were determined by Tukey’s HSD at P<0.001. Differences within the low-Pi series and between the low-Pi and the other two treatments were significant only at 7 d after transfer (P<0.001).