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. 2015 Feb 19;66(9):2475–2485. doi: 10.1093/jxb/erv032

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© The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 6. — Test of phosphorylation of PTL by AKIN10. Mixed extracts of epitope-tagged AKIN10 and PTL proteins were incubated with [γ-³²P]ATP and separated by gel electrophoresis. (A) Autoradiograph showing ³²P-labelling of His–AKIN10-sized bands in lanes 3 and 4, but no labelling of Myc–PTL-sized bands in lanes 2 and 4, indicating that AKIN10 alone has been phosphorylated. (B) Western blot of the same extracts probed with anti-Myc antibody showing the presence of tagged PTL protein in lanes 2 and 4. (C) Western blot of the same extracts probed with anti-His antibody showing the presence of tagged AKIN10 protein in lanes 3 and 4.