(A) Nodal stem content in isopentenyl adenine riboside 5′-monophosphate (iPRMP), zeatin riboside 5′-monophosphate (ZRMP), isopentenyl adenine riboside (iPR), zeatin riboside (ZR), isopentenyl adenine (iP), zeatin (Z), Z O-glucoside (ZOG), and ZR O-glucoside (ZROG) for intact plants at the FBV stage (T0) or grown for 24h on 100mM mannitol, 100mM sucrose, or 30mM palatinose; contents are expressed relative to T0. Relative expression is also shown of (B) RhIPT3 and (C) RhIPT5 in nodal stem sections grown in vitro with 100mM mannitol or 100mM sucrose up to 96h after their excision. Also elongation of buds grown with (D) 30mM sucrose alone or with 10 µM lovastatine, PI-55, or LGR-991; and (E) with 30mM mannitol alone or with 10 µM 6-benzylaminopurine (BAP). Data are mean ± SE of three measurements on a pool of 60 buds (A–C) and 10 replicates (D, E). Asterisks and letters indicate significant differences between the different treatments for each time point.