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. 2015 Mar 7;66(9):2733–2748. doi: 10.1093/jxb/erv069

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© The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 4. — Intracellular co-localization of OsCNIH1 with OsHKT1;3 in plants. (A) Expression of OsCNIH1–mCherry (left) and OsHKT1;3–EYFP (centre), and co-localization of the two proteins (right) in tobacco leaves. (B) Expression of OsCNIH1–mCherry (left) and the ER marker AtWAK2–Citrine (centre), and co-localization of the two proteins (right). (C) Expression of OsHKT1;3–EYFP (centre) and the Golgi marker GmMan1–mCherry (left), and co-localization of the two proteins (right). (D) Scatter plots of pixel distribution of the magenta (y-axis) and green (y-axis) channels employing the Costes algorithm for images shown in (A, left), (B, centre), and (C, right). Scale bar=25 μm.