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. 2015 Mar 7;66(9):2733–2748. doi: 10.1093/jxb/erv069

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© The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 8. — Co-expression of OsHKT1;3 and OsCNIH1 in Xenopus oocytes caused retention of the transporter in the ER, preventing the activation of Na⁺ currents. (A) Sodium inward currents activated by voltage ramps from an oocyte expressing OsHKT1;3 (left). Co-expression of OsHKT1;3 and OsCNIH1 (cRNA ratio injected 1:1) in a Xenopus oocyte (right). (B) Expression of OsHKT1;3–EGFP was observed as puncta at the plasma membrane of a Xenopus oocyte (left); upon co-expression with OsCNIH1, fluorescence was observed exclusively in a reticulated structure (centre). Autofluorescence is from a water-injected oocyte (right). Scale bar=250 μm. (This figure is available in colour at JXB online.)