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. 2015 Mar 7;66(9):2733–2748. doi: 10.1093/jxb/erv069

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© The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 9. — OsCNIH1 restores the intracellular expression of OsHKT1;3 in the yeast mutant BY4741Δerv14 and sensitivity to NaCl. (A) Fluorescence and DIC images of living BY4741 and BY4741Δerv14 cells expressing OsHKT1;3–GFP observed by confocal fluorescence microscopy and co-expressing either OsCNIH1 or ERV14p. Scale bar=5 μm. (B) Drop-test assay on yeast strains BYT4741 (top) and BYT45Δerv14 (bottom) grown in YNB solid medium with different Na⁺ concentrations and transformed with pGRU1 and pDR-F1, OsHKT1;3 and pDR-F1, OsHKT1;3 and OsCNIH1, or OsHKT1;3 and ERV14. Representative results of at least three different experiments are shown.