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. 2015 Mar 18;66(9):2785–2794. doi: 10.1093/jxb/erv094

Fig. 1.

Fig. 1.

Silencing of GFP in P. infestans by hp-RNA. Confocal laser scanning microscopy of P. infestans transformants (Ham34:eGFP) expressing green fluorescent protein. GFP expression in mycelia grown on (A) wild-type and (B) hp-GFPL1 (UBQ:GFP-I-GFP) transgenic potato. Bars=25 μm. (C) Fold change of total intensity of GFP in mycelia grown on hp-GFP plants compared with wild-type plants. AU=arbitrary unit. (D) Transcript abundance of GFP in P. infestans transformants grown on wild-type and transgenic plants at 24, 48, and 72 hpi, quantified by qRT-PCR. Data are normalized to P. infestans actinA mRNA levels and represent means ±SE (n=3 pooled leaves of 3 plants). Asterisks indicate significant difference to the wild type (Student’s t test; *P <0.05; **P <0.01; ***P <0.001). (E) Western blot analysis of GFP protein. hp-GFP plant lines inoculated with: wild type (wt) P. infestans (88069), the GFP-tagged P. infestans, wild-type plants inoculated with: the GFP-tagged P. infestans (+), 88069 (–), and water. Ponceau staining was used as loading control.