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. 2016 Sep;22(9):1386–1399. doi: 10.1261/rna.055798.115

FIGURE 2.

FIGURE 2.

(A) Growth on solid medium of yeast expressing wild-type or mutant forms of the r-proteins expressed from plasmids, at 30°C, 37°C, and 16°C. Empty vectors are included as negative controls for growth. (B) Growth of L7V77E, L35K103-107A and L8Δ1-52, L8Δ1-70 and L8Δ233-256 strains in liquid medium at 30°C, compared to strains expressing the corresponding wild-type proteins from the pRS315 plasmid. Cultures were diluted to ensure exponential growth. OD610 values are plotted as log (ODt/OD0), where ODt is the OD after t hours and OD0 is the starting OD. (C) Incorporation of mutant versions of the ribosomal proteins into pre-ribosomes was tested by TAP-purification of pre-ribosomes followed by Western blot assays. A MYC-tag was used for detection of r-proteins L7 and L35.