Figure 3.

Relative expression level of the SlMLO gene family in response to O. neolycopersici inoculation. Samples were collected at 0, 6, and 10 h after inoculation (hpi). Transcript abundance of each SlMLO homolog was normalized against the transcription level of the 60S ribosomal protein L33 used as reference gene. Bars show standard errors based on four biological replicates. Asterisks refer to significant differences with respect to non-inoculated plants (0 hpi), inferred by mean comparisons with a Student's t-test (^*p < 0.05, ^**p < 0.01, ^***p < 0.001). The SlMLO genes harbored in clade V, based on the phylogenetic tree of Figure 1, are indicated by an arrow spanning their corresponding bars. Similar results were obtained by using the elongation factor Ef1α as housekeeping gene (Supplementary Figure 4).