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Representative Western blot of NOS-1 phosphorylated at Ser1412, and corresponding total NOS-1 content in hearts homogenized before (control, C) and after stimulation with isoproterenol (10nM, 3-min, Iso). The graph shows densitometric analysis of the phospho-NOS-1 over total NOS-1 content ratio (n = 8). No differences were detected.
