Table 1. Hemodynamic parameters in isolated hearts.
| Experimental Group | Pre-Treatment | Post-Treatment | ||
|---|---|---|---|---|
| Basal | Iso | Treatment | Iso | |
| CPP (mmHg) | ||||
| Control (33) | 62.1 ± 3.9 | 53.9 ± 3.4 ** | 67.0 ± 4.1 | 55.0 ± 3.7 (25) *** † |
| SMTC (24) | 53.8 ± 2.8 | 48.2 ± 2.9 *** | 80.9 ± 5.8 ‡ | 61.6 ± 7.9 (16) ** † |
| LNPA (15) | 59.9 ± 5.3 | 56.0 ± 4.1 * | 90.3 ± 7.7 † | 74.9 ± 9.7 (10) * † |
| LNIO (22) | 59.5 ± 3.5 | 59.0 ± 3.0 | 70.5 ± 5.7 † | 65.8 ± 4.6 (14) ¬ |
| Tempol (19) | 48.3 ± 4.8 | 44.0 ± 2.9 * | 71.5 ± 7.4 † | 59.1 ± 5.6 (14) ** † |
| Tempol + SMTC (6) | 48.0 ± 2.8 | 43.7 ± 2.5 ** | 67.3 ± 6.9 † | 55.8 ± 8.5 (6) ** |
| LVDP (mmHg) | ||||
| Control (33) | 77.3 ± 4.3 | 109.8 ± 5.3 *** | 65.4 ± 2.9 ‡ | 100.5 ± 5.8 (25) *** |
| SMTC (24) | 82.1 ± 4.7 | 114.4 ± 6.3 *** | 63.4 ± 3.2 ‡ | 85.8 ± 5.0 (16) † *** |
| LNPA (15) | 72.0 ± 6.3 | 97.9 ± 5.2 *** | 45.5 ± 3.2 ‡ | 70.5 ± 5.1 (10) † *** |
| LNIO (22) | 64.0 ± 3.2 | 113.2 ± 8.4 *** | 66.3 ± 4.0 | 100.1 ± 9.7 (14) *** |
| Tempol (19) | 84.0 ± 6.2 | 114.1 ± 6.7 *** | 59.9 ± 3.7 ‡ | 93.1 ± 6.7 (14) ¬ *** |
| Tempol + SMTC (6) | 65.6 ± 6.2 | 114.4 ± 10.6 ** | 58.0 ± 5.1 | 93.3 ± 8.6 ¬ *** |
| (dP/dt)min mmHg/s] | ||||
| Control (33) | ‒ 1711 ± 106 | ‒ 2848 ± 157 *** | ‒ 1445 ± 86 † | ‒ 2582 ± 154 (25) *** |
| SMTC (24) | ‒ 1945 ± 117 | ‒ 2855 ± 183 *** | ‒ 1340 ± 80 ‡ | ‒ 2098 ± 215 (16) *** ¬ |
| LNPA (15) | ‒ 1995 ± 199 | ‒ 2861 ± 147 *** | ‒ 1132 ± 62 ‡ | ‒ 1864 ± 198 (10) *** ‡ |
| LNIO (22) | ‒ 1264 ± 104 | ‒ 2490 ± 145 *** | ‒ 1283 ± 96 | ‒ 2357 ± 238 (14) *** |
| Tempol (19) | ‒ 1895 ± 124 | ‒ 3106 ± 167 *** | ‒ 1247 ± 76 ‡ | ‒ 1889 ± 182 (14) *** ‡ |
| Tempol + SMTC (6) | ‒ 1329 ± 66 | ‒ 2876 ± 312 ** | ‒ 1303 ± 78 | ‒ 2459 ± 338 (6) ** ¬ |
| (dP/dt)max, (mmHg/s) | ||||
| Control (33) | 2592 ± 113 | 4170 ± 181 *** | 2316 ± 111 † | 4302 ± 288 (25) *** |
| SMTC (24) | 2608 ± 114 | 4094 ± 188 *** | 1889 ± 101 ‡ | 2806 ± 248 (16) † ** |
| LNPA (15) | 2771 ± 192 | 4495 ± 231 *** | 1971 ± 132 † | 2692 ± 334 (10) ‡ *** |
| LNIO (22) | 2484 ± 116 | 4657 ± 292 *** | 2770 ± 114 ¬ | 4812 ± 283 (11) *** |
| Tempol (19) | 2788 ± 149 | 4132 ± 210 *** | 1930 ± 118 ‡ | 2753 ± 219 (14) ‡ *** |
| Tempol + SMTC (6) | 2144 ± 143 | 3934 ± 272 ** | 1989 ± 177 | 3119 ± 308 ¬ *** |
Table 1 summarizes the values of Coronary Perfusion Pressure (CCP); Left Ventricular Developed Pressure (LVDP); Maximal rate of pressure relaxation ((dP/dt)min); and Maximal rate of pressure development ((dP/dt)max), for all hearts included in this study. In all conditions, heart rate was kept constant, at 300 BPM. The first 2 columns (Pre-Treatment) show values at the end of a 20-min equilibration period (Basal) and after a 3-min stimulus with 10nM Isoproterenol (Iso). Hearts were allowed a 10-min period of washout, continued with 20-min perfusion with one inhibitor, or a combination of inhibitors, or vehicle, as indicated in the Experimental Group category (Post-Treatment), which was followed by a second challenge with 10nM Isoproterenol in the presence of the inhibitor (Iso). The number of hearts in each group is shown in parenthesis, next to the group’s name for columns 1–3, or within the fourth column. The fourth column has lower numbers because some hearts were homogenized for biochemical studies before the second stimulation.
* = p<0.05
** = p<0.001
*** = p<0.0001
Iso vs. precedent basal value, (paired t test)
¬ = p<0.05
† = p<0.01
‡ = p<0.001 vs. respective Pre-Treatment value (paired t test)