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. 2016 Aug 16;12(8):e1005782. doi: 10.1371/journal.ppat.1005782

Fig 8. Effects of treating both CD4 T cells and NK cells with HDACi depends on the chosen HDACi.

Fig 8

A) An NK co-culture assay was performed overnight at a 10:1:1 E:T:NT ratio with either infected, untreated CD4 T (targets) and untreated NK cells (effectors) or with 20nM panobinostat treated targets and HDACi treated effectors. Intracellular p24 was measured and compared between the samples with and without NK cells. The difference in p24 expression was then converted to percent killing as described. A Wilcoxon matched-pairs signed rank test was performed (n = 5). In B, a similar co-culture assay was performed using 2 doses of vorinostat (333nM and 50nM), 2 doses of panobinostat (20nM and 5nM), 2 doses of romidepsin (10nM and 5nM) and 300nM prostratin. A Friedman test with Dunn’s multiple comparison test was performed (n = 5). Different colors represent different donors.