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. 2016 Aug 16;11(8):e0161236. doi: 10.1371/journal.pone.0161236

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© 2016 Ying et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 7 — (A) Alignment of Rab8a and Rab8b amino acid sequences. Exon 1 contains the GTP binding domain. Exon 2 is deleted in the germline and conditional knockouts. T22, preventing GTP binding when mutated, is shown. (B) Genotyping confirms deletion of exon2 in ^retRab8a^-/- retinas but not tails. ^retRab8a^+/- tissues were used as controls. (C) Immunofluorescence showed that Rab8a is mainly localized as punctate signal in IS and OPL in WT but absent in ^retRab8a^-/- photoreceptor cells. (D) Rab8b(T22N) is mainly localized at IS and OPL. (E) Rhodopsin, GC1, CNGA1/A3 and ribeye are localized correctly in 4M-old ^retRab8a^-/- and ^retRab8a^+/- animals injected with dnRab8b (Rab8b(T22N)) AAV. Scale bars, 20 μm (C and D) and 30 μm (E).