Table 3. Concentrations of plasma metabolites at different time points, and individual, pairwise fold-change between them.
| Compound | ppm | Median concentrations (IQR) in mM | Pairwise FC (p- and q-value) | |||
|---|---|---|---|---|---|---|
| t1 | t2 | t3 | t1 to t2 | t2 to t3 | ||
| Acetate | 1.91 | 0.0053 (0.000061–0.018) | 0.014 (0.00023–0.087) | 0.014 (0.0028–0.1) | 2.82 (1.5e-09; 2.67e-09) | 1.6 (0.0033; 0.0056) |
| Alanine | 1.48 | 0.64 (0.37–1.0) | 1.5 (0.64–2.6) | 1.2 (0.43–2.6) | 2.21 (4.4e-37; 7.04e-36) | -1.38 (2.5e-11; 1.6e-10) |
| Choline | 3.19 | 0.013 (0–0.049) | 0.061 (0.012–0.14) | 0.077 (0.021–0.22) | 4.85 (1.8e-26; 5.24e-26) | 1.22 (5.7e-05; 1.3e-04) |
| Creatinine | 4.05 | 0.034 (0.023–0.05) | 0.045 (0.022–0.063) | 0.061 (0.038–0.082) | 1.29 (8.4e-15; 1.68e-14) | 1.27 (5.7e-14; 9.12e-13) |
| DMA | 2.72 | 0.0078 (0.0042–0.012) | 0.0088 (0.0048–0.012) | 0.0099 (0.0058–0.013) | 1.15 (4.2e-05; 5.84e-05) | 1.09 (0.011; 0.17) |
| Formate | 8.45 | 0.0083 (0–0.043) | 0.012 (0–0.054) | 0.018 (0–0.035) | 1.4 (0.078; 0.86) | 1.81 (0.00019; 0.00038) |
| Fumarate | 6.51 | 0.0056 (0.0017–0.013) | 0.033 (0.0047–0.083) | 0.019 (0.0012–0.085) | 4.85 (3.9e-33; 3.12e-32) | -1.56 (1.1e-06; 3.2e-06) |
| Glucose | 5.24 | 6 (4.1–8.6) | 11 (5.5–20) | 6.6 (2.7–16) | 1.77 (4.3e-32; 2.29e-31) | -1.53 (8.8e-14; 9.39e-13) |
| Glutamine | 2.45 | 0.27 (0.19–0.4) | 0.45 (0.25–0.76) | 0.51 (0.25–1.0) | 1.65 (1.9e-32; 1.22e-31) | 1.06 (0.055; 0.074) |
| Glycerol | 3.57 | 0.18 (0.0041–0.39) | 0.35 (0.045–1.0) | 0.2 (0.018–2.2) | 1.8 (3.5e-06; 5.09e-06) | -1.2 (0.06; 0.08) |
| Glycine | 3.55 | 0.61 (0.39–1.0) | 0.95 (0.39–1.5) | 0.89 (0.43–1.7) | 1.44 (1.3e-23; 3.2e-23) | 1.03 (0.56; 0.60) |
| Hypoxanthine | 8.20 | 0.041 (0.023–0.068) | 0.12 (0.036–0.26) | 0.06 (0.017–0.27) | 2.63 (1.0e-27; 3.2e-27) | -1.6 (3.8e-09; 1.52e-08) |
| Isoleucine | 1.00 | 0.097 (0.061–0.16) | 0.1 (0.055–0.2) | 0.065 (0.044–0.18) | 1.06 (0.15; 0.16) | -1.39 (3.5e-11; 1.9e-10) |
| Lactate | 1.33 | 2.9 (1.5–5.3) | 10 (3.7–16) | 5.3 (1.3–16) | 3.07 (2.1e-38; 6.72e-37) | -1.75 (2.1e-16; 6.7e-15) |
| Leucine | 0.95 | 0.11 (0.074–0.17) | 0.14 (0.054–0.32) | 0.089 (0.053–0.29) | 1.23 (0.00024; 0.00032) | -1.37 (5e-10; 2.3e-9) |
| Malate | 2.65 | 0.013 (0–0.062) | 0.13 (0.013–0.28) | 0.088 (0.02–0.29) | 6.69 (3.0e-20; 6.4e-20) | -1.07 (0.35; 0.40) |
| Methionine | 2.63 | 0.032 (0.0019–0.077) | 0.032 (0.0045–0.087) | 0.03 (0–0.088) | 1.02 (0.79; 0.79) | -1.22 (0.05; 0.07) |
| Myo-inositol | 4.06 | 0.064 (0–0.39) | 0.2 (0.045–0.6) | 0.23 (0.037–0.6) | 2.26 (1.6e-12; 3.01e-12) | 1.02 (0.71; 0.71) |
| Phenylalanine | 7.33 | 0.051 (0.026–0.079) | 0.064 (0.018–0.14) | 0.046 (0.018–0.13) | 1.22 (9e-04; 1.15e-03) | -1.27 (1.0e-06; 3.2e-06) |
| Proline | 4.14 | 0.49 (0.32–0.84) | 0.57 (0.34–0.92) | 0.57 (0.32–0.94) | 1.08 (0.0022; 0.0027) | -1.09 (0.00075; 0.0014) |
| Pyruvate | 2.37 | 0.1 (0.059–0.21) | 0.21 (0.11–0.34) | 0.2 (0.058–0.33) | 2.0 (1.5e-29; 5.33e-29) | -1.22 (0.00083; 0.0015) |
| Succinate | 2.40 | 0.015 (0.0054–0.035) | 0.17 (0.0095–0.62) | 0.072 (0.0035–0.44) | 7.67 (1.2e-30; 5.49e-30) | -1.54 (4.7e-05; 1.16e-04) |
| Trimethylamine | 2.88 | 0.0011 (0–0.0033) | 0.0046 (0.00083–0.016) | 0.0054 (0.00092–0.019) | 4.43 (4.7e-34; 5.01e-33) | 1.26 (0.0097; 0.016) |
| Tyrosine | 7.20 | 0.11 (0.059–0.2) | 0.13 (0.066–0.22) | 0.11 (0.047–0.19) | 1.21 (1.6e-06; 2.44e-06) | -1.22 (6.1e-09; 2.17e-08) |
| Valine | 1.03 | 0.19 (0.11–0.34) | 0.22 (0.082–0.45) | 0.16 (0.069–0.36) | 1.08 (0.12; 0.13) | -1.36 (1.4e-11; 1.12e-10) |
| Unknown doublet | 1.06 | 1.6 (0.094–4.5) | 2.1 (0.0015–5.1) | 1.9 (0.7–4.6) | 1.34 (0.012; 0.015) | -1.04 (0.66; 0.68) |
| Unknown doublet | 1.11 | 2.7 (1.8–3.8) | 2.2 (0.33–3.7) | 2.4 (1.1–3.9) | -1.59 (2.1e-07; 3.36e-07) | 1.09 (0.22; 0.27) |
| Unknown doublet | 1.14 | 0.19 (0.12–0.47) | 0.14 (0.083–0.43) | 0.14 (0.063–0.28) | -1.27 (1.4e-08; 2.36e-08) | -1.07 (0.034; 0.05) |
| Unknown multiplet | 1.40 | 40 (24–63) | 23 (9.1–43) | 25 (9.4–43) | -1.92 (1.2e-29; 4.8e-29) | -1.06 (0.46; 0.51) |
| Unknown singlet | 3.92 | 2.7 (1.3–7.7) | 3.3 (0.8–14) | 3.4 (1.4–25) | 1.14 (0.025; 0.029) | 1.3 (0.00013; 0.00028) |
| Unknown singlet | 5.39 | 1.1 (0.24–2.8) | 1.9 (0.25–6) | 2.6 (0.34–8) | 1.78 (6.6e-23; 1.51e-22) | 1.16 (8e-06; 2.2e-05) |
| FWHM of TSP | 0.0047 (0.003–0.007) | 0.004 (0.0026–0.0052) | 0.0038 (0.0027–0.0051) | -1.24 (6.1e-25; 1.63e-24) | -1.02 (0.26; 0.31) | |
Time point t1: baseline, between surgical preparation and asphyxiation; t2: 2 hours of rest after resuscitation; t3: 4 hours of rest. Concentrations are reported in mM, except for the unidentified signals; their values are in arbitrary units proportional to actual concentrations. FWHM (full width at half maximum) of the TSP signal is affected by plasma lipoproteins and is reported in spectral ppm.