Table 1.

Sequences of primers used for PCR amplification.

Target	Primer	Primer sequence	Size (bp)	Accession number (Genbank)
TH	Forward	5′ ccg cac att tgc cca gtt c 3′	148	NM_009377.1
	Reverse	5′ tgc acc gta agc ctt cag ctc 3′
Nurr1	Forward	5′ gac ggg ctg gat tcc caa tag 3′	157	NM_001139509.1
	Reverse	5′ cct tga ggc gag gac cca ta 3′
Lmx1b	Forward	5′ gga agg tcc gag aga cat tgg 3′	125	NM_010725.2
	Reverse	5′ ctg ctc ctg ctg ttg ctg gtg t 3′
Lmx1a	Forward	5′ cag tgt gcc tcc tgc aaa gag 3′	137	NM_033652.5
	Reverse	5′ aac tca ttg ggc gca atg g 3′
Wnt1	Forward	5′ gct gtg cga gag tgc aaa tgg 3′	133	NM_021279.4
	Reverse	5′ cgg agg tga ttg cga aga tg 3′
Ngn2	Forward	5′ aag aag acc cgc agg ctc aag 3′	158	NM_009718.2
	Reverse	5′ cag atg taa ttg tgg gcg aag c 3′
Pitx3	Forward	5′ tgt cgt tat cgg acg cag g 3′	139	NM_008852.4
	Reverse	5′ tgc ttc ttc ttc aga gag ccg tc 3′
β-actin	Forward	5′ tga gag gga aat cgt gcg aga c 3′	149	NM_007393.3
	Reverse	5′ gct cgt tgc caa tag tga tga cc 3′

TH – tyrosine hydroxylase; Nurr1 – nuclear receptor related-1 protein; Lmx1a/Lmx1b – LIM homeobox transcription factor 1 alpha/1 beta; Wnt1 – Wingless type MMTV integration site family member 1; Ngn2 – neurogenin 2; Pitx3 – paired-like homeodomain transcription factor 3.