Table 2.
Parasite concentrations (per ml) in finger prick blood of volunteers from northern Ethiopia estimated by qRT-kDNA PCR. Results are compared with the corresponding results obtained by the different LAMP assays. Positive LAMP results are designated by the take-off time in mins (see materials and methods).
| Sample number | History of VL | Parasite/ml qRT-kDNA-PCR |
Estimated take-off time of LAMP amplification (min) |
||
|---|---|---|---|---|---|
| LITS- LAMP1 | LITS- LAMP2 | L151 LAMP | |||
| 1 | + | 0 | – | – | 61 |
| 2 | – | 0 | – | – | – |
| 3 | – | 0 | – | – | – |
| 4 | – | 0 | – | – | – |
| 5 | – | 0 | – | – | – |
| 6 | + | 0 | – | – | 67 |
| 7 | – | 0 | – | – | – |
| 8 | – | 0 | – | – | – |
| 9 | – | 0 | – | – | – |
| 10 | – | 0 | – | – | – |
| 11 | + | 18 | 69 | 57 | – |
| 12 | – | 0 | – | – | – |
| 13 | – | 0 | – | – | – |
| 14 | – | 0 | – | – | – |
| 15 | – | 0 | – | – | – |
| 16 | + | 0 | – | – | – |
| 17 | – | 0 | – | – | – |
| 18 | – | 0 | – | – | – |
| 19 | – | 0 | – | – | – |
| 20 | – | 3 | – | – | – |
| 21 | + | 11 | – | 78 | 63 |
| 22 | – | 595 | 59 | 54 | 52 |
| 23 | – | 0 | – | – | 86 |
| 24 | – | 59 | – | 85 | 65 |
| 25 | – | 0 | – | – | – |
| 26 | + | 0 | – | – | – |
| 27 | – | 0 | – | 72 | 86 |
| 28 | – | 0 | – | 62 | 65 |
| 29 | – | 0 | – | 48 | – |
| 30 | – | 0 | – | 48 | 103 |
| 31 | + | 0 | – | 41 | 34 |
| 32 | – | 0 | – | 57 | – |
| 33 | – | 0 | – | 47 | – |
| 34 | – | 9 | – | – | – |
| 35 | – | 0 | – | – | – |
| 36 | + | 2 | 75 | 41 | 34 |
| 37 | – | 0 | – | 59 | – |
| 38 | – | 1 | – | 43 | – |
| 39 | – | 0 | – | – | – |
| 40 | + | 0 | 56 | 44 | 58 |
| 41 | – | 0 | 60 | 54 | – |
| 42 | – | 23 | – | 42 | 52 |
| 43 | – | 0 | 60 | 53 | – |
| 44 | – | 0 | – | 45 | – |