Fig. 1.

Dose–dependent binding of gC1qR to HCV core protein.

Microtiter platewells were first coated with 2 μg/ml HCV core protein (1 h, 37 °C). After washing and blocking with 5% non-fat dry milk, the bound HCV core protein was reacted (overnight, 4C) with concentrations of biotinylated WT gC1qR ranging from 0 to 5 μg/ml followed by sequential reaction with alkaline-phosphatase conjugated streptavidin and pNPP following the standard procedure for ELISA. Each data point is a mean ± SD of duplicate experiments and the figure is a representative of three such experiments.