Fig. 3.

Binding of gC1qR to the HIV-1 envelope glycoprotein, gp41.

ELISA plate wells were first coated with 100 μl per well of 2 μg/ml of gp41 (1 h, 37 °C). Following blocking with 5% non-fat dry milk for 1 h, wells were washed (3× with TBS-T) and incubated with concentrations of gC1qR (1 μg, 0.5 μg and 0.25 μg/ml) in TBS, (1 h, 37 °C). Bound gC1qR was then detected using mAb 74.5.2 anti-gC1qR (0.5 μg/ml) followed by sequential reaction with alkaline phosphatase goat anti-mouse IgG and pNPP. The absorbance of the color developed after 30 min was measured spectrophotometrically at 405 nm. Each data bar is a mean ± SD of each experiment run in duplicates (n = 3).