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. Author manuscript; available in PMC: 2016 Aug 16.
Published in final edited form as: Mol Immunol. 2016 Apr 23;74:18–26. doi: 10.1016/j.molimm.2016.03.016

Fig. 6.

Fig. 6

Inhibition of gp41-3S peptide binding to MOLT-4 cells by gC1qR peptides.

First the binding of gp41-3S peptide to MOLT-4 cells was verified using microtiter plate fixed MOLT-4 cells (A) as described in detail in Material and Methods. The bound gp41-3S peptide was detected using rabbit anti-gp41-3S antibody. Then the ability of selected gC1qR peptides (corresponding to the deleted regions) to inhibit the binding of gp41-3S peptide to MOLT-4 cells was carried out by preincubation of 100 μl of 5 μg/ml of each peptide with MOLT-4 cells (O/N, 4 °C). Then the unbound gC1qR peptides were removed and the wells further incubated with gp41-3S peptide (1 h, room temp). After incubation the bound gp41-3S peptide was detected with affinity purified anti-gp41-3S peptide, and the reaction developed following the protocol for a standard ELISA (B). For visual clarity, the location of one of the inhibitory peptides (aa204-218) within the gC1qR 3D structure is shown in (C). The significance of the data is indicated as: **p ≤ 0.01; n = 3.