Figure 1. BubR1 transgenic mutant proteins are highly overexpressed in vitro and in vivo.
(A) (top) Bub1b transgenic vector design. pCAGGS, promoter consisting of the CMV immediate enhancer and the chicken-actin promoter. FL, full-length. IRES, internal ribosome entry site. (bottom) Schematics of the Flag-Bub1b transgenic mouse constructs. KEN, KEN-box. D, destruction-(D-)box. GLEBs, GLEBs-binding motif. Phe, Phe box. KARD, kinetochore attachment regulatory domain. (B) Western blots of MEF (left) and lung tissue extracts (right) from wild-type (WT) and Flag-Bub1b transgenic mice. Blots were probed with the indicated antibodies. Ponceau S was used to normalize loading.
Figure 1—figure supplement 1. Analysis of BubR1 overexpression in transgenic MEFs.
Full scan and multiple exposures of Western blots of MEF lysates from Figure 1B. WT, wild-type. FL, full-length.