Figure 8. Schematics of pTripZ-Flag-Bub1b mutants.
Schematics of the pTripZ-Flag-Bub1b constructs. D, destruction-(D-)box. GLEBs, GLEBs-binding motif. Phe, Phe box. KARD, kinetochore attachment regulatory domain. FL, full-length. Sequence alignment of the Phe box, D-box2, and KARD region of human and mouse BubR1. Residues characterized in human BubR1 are underlined, and homologous residues deleted in mouse Bub1b constructs are bold.
Figure 8—figure supplement 1. Protein levels of Bub1b deletion constructs in wild-type MEFs.
Western blots of wild-type (WT) MEFs infected with the indicated constructs with or without doxycycline (Dox). Blots were probed with the indicated antibodies. Ponceau S was used to normalize loading. Asterisk indicates specific band for BubR1ΔI.