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(a) A functional GFP::DDI-1 fusion protein is strongly induced and localizes to the nucleus upon proteasome disruption by rpt-5(RNAi). Scale bar 20 µm. (b) Western blot showing induction of HA-tagged endogenous DDI-1 upon proteasome disruption by bortezomib. Actin is used as a loading control. (c) Induction of GFP::DDI-1 upon proteasome disruption by rpt-5(RNAi) is lost in skn-1a mutants. Scale bar 20 µm.
