Table 1.
EMS-induced mutations that disrupt rpt-3::gfp activation.
| Allele | Affected gene | Effect | Homologues |
|---|---|---|---|
| mg563 | C01G5.6 | L245F | DDI1. Aspartic protease. |
| mg555 | C01G5.6 | C277S | |
| mg544 | C01G5.6 | G293R | |
| mg542 | C01G5.6 | R350STOP | |
| mg543 | C01G5.6 | M244I | |
| mg557 | C01G5.6 | L334F | |
| mg565 | sel-1 | G594E | HRD3/SEL1. ER membrane protein, required for ERAD substrate recognition. |
| mg567 | sel-1 | A522T | |
| mg547 | sel-1 | splice site | |
| mg550 | sel-9 | S140F | EMP24/TMED2. ER membrane protein. |
| mg561 | png-1 | G498R | PNG1/NJLY1. Peptide N-glycanase. Removes N-linked glycans during ERAD. |
| mg564 | png-1 | splice site |