Figure 2. PTRF mediated ribosomal transcription regulations.

(A) Relative pre-rRNA (47S) levels were detected by RT-qPCR from 3T3-L1 fibroblast and fully differentiated adipocytes stimulated by switching culture medium from 1% BSA in PBS to full growth medium with insulin (Fed). (B) Basal (KRP buffer with 1% BSA) or 45 min stimulated (Fed: high glucose DMEM, 10% FBS, and insulin) pre-rRNA levels were detected by RT-qPCR in primary adipocytes isolated from wild type (WT) and PTRF null (KO) mice. (C) A stimulation time-course of B. (D) Pre-rRNA levels were detected by RT-qPCR from basal or stimulated wild type (WT), PTRF null (KO), PTRF re-transfected PTRF null (KO+PTRF), and Cav-1 re-transfected PTRF null (KO+Cav1) MEFs cells. (E) Relative levels of total proteins, RNA, 18S, and 47S from wild type or PTRF null mice (n = 6) were measured and normalized to cell numbers. *p<0.05, **p<0.01, and ***p<0.001; Student’s test. Error bars indicate SD.

DOI: http://dx.doi.org/10.7554/eLife.17508.004

Figure 2—figure supplement 1. Characterization of ribosome biogenesis in 6–8 weeks old PTRF null and control mice.

Total proteins, RNAs, 18S, and 47S levels were measured from 6–8 weeks old wild type control and PTRF null mice (n = 6).

DOI: http://dx.doi.org/10.7554/eLife.17508.005

Figure 2—figure supplement 2. Cell growth curve of cultured WT and PTRF null MEFs cells.

Primary PTRF null (KO) and wild type control (WT) MEFs cell numbers were determined by cell counting on indicated days. **p<0.01; Student’s test. Error bars indicate SD.

DOI: http://dx.doi.org/10.7554/eLife.17508.006